Neuroblastoma (NB) is the most common extracranial tumour in children and poses significant therapeutic challenges, with only a 40% long-term survival rate for advanced stages. Despite aggressive chemotherapy and retinoid-mediated differentiation, advanced NB remains difficult to treat, partly due to the role of treatment-resistant cancer stem cells (CSCs) and the complex regulation of the cell cycle. Recent research highlights that CSCs can evade therapy and drive tumour progression through dynamic cell cycle changes. By developing advanced computer-based tools and novel 3D organoid models with live-cell imaging, unique insights into targeting cell cycle dynamics for improving treatment outcomes can be explored. The findings are expected to address critical gaps in current tumour models and provide new strategies for more effective NB treatment. 

By developing advanced tools to study cell cycle dynamics in NB, this research promises to enhance treatment strategies and improve patient outcomes. The integration of novel 3D organoid models and live-cell imaging will offer unprecedented insights into tumour biology, potentially revolutionizing therapeutic approaches and advancing fundamental cancer research.